Establishment of a new protein C detection system based on chromogenic substrate assay and its clinical diagnostic value for deep vein thrombosis.

ABSTRACT:

Background

Deficiency of protein C (PC) affects the balance between blood coagulation and fibrinolysis in the human body. Chromogenic-based assay is recommended as the preferred screening method for detecting PC deficiency. We established a PC detection system based on the chromogenic substrate assay.

Methods

First, a kit for the determination of PC activity in plasma was elaborately developed and its reaction parameters on XL-3200c were explored. Then, we evaluated its performance and collected specimens to compare the test results obtained with those of the Siemens detection system. Finally, the clinical diagnostic efficacy of this detection system for deep vein thrombosis (DVT) was assessed.

Results

Optimum conditions for PC detection were 0.25-0.1 U/ml protein C a

SUBMITTER: Lu W 

PROVIDER: S-EPMC8649361 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature