Cell-type-specific regulation of the retinoic acid receptor mediated by the orphan nuclear receptor TLX.

ABSTRACT: Malformations in the eye can be caused by either an excess or deficiency of retinoids. An early target gene of the retinoid metabolite, retinoic acid (RA), is that encoding one of its own receptors, the retinoic acid receptor beta (RARbeta). To better understand the mechanisms underlying this autologous regulation, we characterized the chick RARbeta2 promoter. The region surrounding the transcription start site of the avian RARbeta2 promoter is over 90% conserved with the corresponding region in mammals and confers strong RA-dependent transactivation in primary cultured embryonic retina cells. This response is selective for RAR but not retinoid X receptor-specific agonists, demonstrating a principal role for RAR(s) in retina cells. Retina cells exhibit a far higher sensitivity to RA than d

SUBMITTER: Kobayashi M 

PROVIDER: S-EPMC86495 | biostudies-literature | 2000 Dec

REPOSITORIES: biostudies-literature