SPAK Deficiency Attenuates Chemotherapy-Induced Intestinal Mucositis.

ABSTRACT:

Introduction

Ste20-related protein proline/alanine-rich kinase (SPAK) affects cell proliferation, differentiation, and transformation, and sodium and chloride transport in the gut. However, its role in gut injury pathogenesis is unclear.

Objective

We determined the role of SPAK in chemotherapy-induced intestinal mucositis using in vivo and in vitro models.

Methods

Using SPAK-knockout (KO) mice, we evaluated the severity of intestinal mucositis induced by 5-fluorouracil (5-FU) by assessing body weight loss, histological changes in the intestinal mucosa, length of villi in the small intestine, pro-inflammatory cytokine levels, proliferative indices, and apoptotic indices. We also evaluated changes in gut permeability and tight junction-associated protein e

SUBMITTER: Huang TY 

PROVIDER: S-EPMC8649624 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature