HPMSCs-Derived Exosomal miRNA-21 Protects Against Aging-Related Oxidative Damage of CD4⁺ T Cells by Targeting the PTEN/PI3K-Nrf2 Axis.

ABSTRACT: Mesenchymal stem cells (MSCs)-derived exosomes were considered a novel therapeutic approach in many aging-related diseases. This study aimed to clarify the protective effects of human placenta MSCs-derived exosomes (hPMSC-Exo) in aging-related CD4⁺ T cell senescence and identified the underlying mechanisms using a D-gal induced mouse aging model. Senescent T cells were detected SA-β-gal stain. The degree of DNA damage was evaluated by detecting the level of 8-OH-dG. The superoxide dismutase (SOD) and total antioxidant capacity (T-AOC) activities were measured. The expression of aging-related proteins and senescence-associated secretory phenotype (SASP) were detected by Western blot and RT-PCR. We found that hPMSC-Exo treatment markedly decreased oxidative stress damage (ROS and

SUBMITTER: Xiong Y 

PROVIDER: S-EPMC8649962 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature