An improved procedure for isolating adult mouse cardiomyocytes for epicardial activation mapping.

ABSTRACT: Cardiovascular disease is a leading cause of death and disability worldwide. Although genetically modified mouse models offer great potential for robust research in vivo, in vitro studies using isolated cardiomyocytes also provide an important approach for investigating the mechanisms underlying cardiovascular disease pathogenesis and drug actions. Currently, isolation of mouse adult cardiomyocytes often relies on aortic retrograde intubation under a stereoscopic microscope, which poses considerable technical barriers and requires extensive training. Although a simplified, Langendorff-free method has been used to isolate viable cardiomyocytes from the adult mouse heart, the system requires enzymatic digestions and continuous manual technical operation. This study established an optimized a

SUBMITTER: Zhang Z 

PROVIDER: S-EPMC8650026 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature