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Femtomolar and locus-specific detection of N⁶-methyladenine in DNA by integrating double-hindered replication and nucleic acid-functionalized MB@Zr-MOF.

ABSTRACT: In this study, a novel electrochemical biosensor was constructed for ultrasensitive and locus-specific detection of N⁶-Methyladenine (m⁶A) in DNA using double-hindered replication and nucleic acid-coated methylene blue (MB)@Zr-MOF. Based on the combination of m⁶A-impeded replication and Ag^I-mediated mismatch replication, this mode could effectively stop the extension of the strand once DNA polymerase encountered m⁶A site, which specifically distinguish the m⁶A site from natural A site in DNA. Also, Zr-MOF with high porosity and negative surface potential features was carefully chose to load cationic MB, resulting a stable and robust MB@Zr-MOF electrochemical tag. As a result, the developed biosensor exhibited a wide linear r

SUBMITTER: Zheng Q

PROVIDER: S-EPMC8650346 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature

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Femtomolar and locus-specific detection of N⁶-methyladenine in DNA by integrating double-hindered replication and nucleic acid-functionalized MB@Zr-MOF.

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Femtomolar and locus-specific detection of N6-methyladenine in DNA by integrating double-hindered replication and nucleic acid-functionalized MB@Zr-MOF.

OmicsDI is part of the ELIXIR infrastructure

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Femtomolar and locus-specific detection of N⁶-methyladenine in DNA by integrating double-hindered replication and nucleic acid-functionalized MB@Zr-MOF.