From single-molecule to genome-wide mapping of DNA lesions: repair-assisted damage detection sequencing.

ABSTRACT: Mapping DNA damage and its repair has immense potential in understanding environmental exposures, their genotoxicity, and their impact on human health. Monitoring changes in genomic stability also aids in the diagnosis of numerous DNA-related diseases, such as cancer, and assists in monitoring their progression and prognosis. Developments in recent years have enabled unprecedented sensitivity in quantifying the global DNA damage dose in cells via fluorescence-based analysis down to the single-molecule level. However, genome-wide maps of DNA damage distribution are challenging to produce. Here, we describe the localization of DNA damage and repair loci by repair-assisted damage detection sequencing (RADD-seq). Based on the enrichment of damage lesions coupled with a pull-down assay and foll

SUBMITTER: Gilat N 

PROVIDER: S-EPMC8651515 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature