Long-term STED imaging of membrane packing and dynamics by exchangeable polarity-sensitive dyes.

ABSTRACT: Understanding the plasma membrane nanoscale organization and dynamics in living cells requires microscopy techniques with high spatial and temporal resolution that permit for long acquisition times and allow for the quantification of membrane biophysical properties, such as lipid ordering. Among the most popular super-resolution techniques, stimulated emission depletion (STED) microscopy offers one of the highest temporal resolutions, ultimately defined by the scanning speed. However, monitoring live processes using STED microscopy is significantly limited by photobleaching, which recently has been circumvented by exchangeable membrane dyes that only temporarily reside in the membrane. Here, we show that NR4A, a polarity-sensitive exchangeable plasma membrane probe based on Nile red, permi

SUBMITTER: Carravilla P 

PROVIDER: S-EPMC8651516 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature