Mitochondrial matrix protein C14orf159 attenuates colorectal cancer metastasis by suppressing Wnt/β-catenin signalling.

ABSTRACT:

Background

The mechanisms underlying metastasis of colorectal cancer (CRC) remain unclear. C14orf159 is a mitochondrial matrix protein converting D-glutamate to 5-oxo-D-proline. Other metabolic functions of C14orf159, especially on mitochondrial metabolism, and its contribution to CRC metastasis, are not elucidated.

Methods

Metabolome analysis by gas chromatography-mass spectrometry, RNA-sequencing analysis, flow cytometry, migration and invasion assay, sphere-formation assay using C14orf159-knockout and -stable expressing cells, immunohistochemistry of C14orf159 in human CRC specimens, and xenograft experiments using Balb/c nude mice were conducted.

Results

C14orf159 maintained the mitochondrial membrane potential of human CRC cells, and its involvement in amino acid

SUBMITTER: Ohshima K 

PROVIDER: S-EPMC8651639 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature