Discovery of indole-modified aptamers for highly specific recognition of protein glycoforms.

ABSTRACT: Glycosylation is one of the most abundant forms of post-translational modification, and can have a profound impact on a wide range of biological processes and diseases. Unfortunately, efforts to characterize the biological function of such modifications have been greatly hampered by the lack of affinity reagents that can differentiate protein glycoforms with robust affinity and specificity. In this work, we use a fluorescence-activated cell sorting (FACS)-based approach to generate and screen aptamers with indole-modified bases, which are capable of recognizing and differentiating between specific protein glycoforms. Using this approach, we were able to select base-modified aptamers that exhibit strong selectivity for specific glycoforms of two different proteins. These aptamers can discri

SUBMITTER: Yoshikawa AM 

PROVIDER: S-EPMC8651674 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature