Ontology highlight
ABSTRACT:
SUBMITTER: Wu L
PROVIDER: S-EPMC8652005 | biostudies-literature | 2021 Dec
REPOSITORIES: biostudies-literature
Wu Lin L Chen Dian D Ding Jienyu J Liu Yu Y
iScience 20211125 12
RNAs adopt various conformations to perform different functions in cells. Incapable of acquiring intermediates, the key initiations of ligand recognition in the adenine riboswitch have not been characterized. In this work, stopped-flow fluorescence was used to track structural switches in the full-length adenine riboswitch in real time. We used PLOR (position-selective labeling of RNA) to incorporate fluorophores into desired positions in the RNA. The switching sequence P1 responded to adenine m ...[more]