Host Lung Environment Limits Aspergillus fumigatus Germination through an SskA-Dependent Signaling Response.

ABSTRACT: Aspergillus fumigatus isolates display significant heterogeneity in growth, virulence, pathology, and inflammatory potential in multiple murine models of invasive aspergillosis. Previous studies have linked the initial germination of a fungal isolate in the airways to the inflammatory and pathological potential, but the mechanism(s) regulating A. fumigatus germination in the airways is unresolved. To explore the genetic basis for divergent germination phenotypes, we utilized a serial passaging strategy in which we cultured a slow germinating strain (AF293) in a murine-lung-based medium for multiple generations. Through this serial passaging approach, a strain emerged with an increased germination rate that induces more inflammation than the parental strain (herein named LH-EVOL for l

SUBMITTER: Kirkland ME 

PROVIDER: S-EPMC8653827 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature