A workflow for rapid unbiased quantification of fibrillar feature alignment in biological images.

ABSTRACT: Measuring the organisation of the cellular cytoskeleton and the surrounding extracellular matrix (ECM) is currently of wide interest as changes in both local and global alignment can highlight alterations in cellular functions and material properties of the extracellular environment. Different approaches have been developed to quantify these structures, typically based on fibre segmentation or on matrix representation and transformation of the image, each with its own advantages and disadvantages. Here we present AFT-Alignment by Fourier Transform, a workflow to quantify the alignment of fibrillar features in microscopy images exploiting 2D Fast Fourier Transforms (FFT). Using pre-existing datasets of cell and ECM images, we demonstrate our approach and compare and contrast this workflow w

SUBMITTER: Marcotti S 

PROVIDER: S-EPMC8654057 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature