Accurate Identification of Degraded Products of Aflatoxin B₁ Under UV Irradiation Based on UPLC-Q-TOF-MS/MS and NMR Analysis.

ABSTRACT: Analysis, purification, and characterization of AFB₁ degraded products are vital steps for elucidation of the photocatalytic mechanism. In this report, the UPLC-Q-TOF-MS/MS technique was first coupled with purification and NMR spectral approaches to analyze and characterize degraded products of AFB₁ photocatalyzed under UV irradiation. A total of seventeen degraded products were characterized based on the UPLC-Q-TOF-MS/MS analysis, in which seven ones (1-7) including four (stereo) isomers (1,2, 5, and 6) were purified and elucidated by NMR experiments. According to the structural features of AFB₁ and degraded products (1-7), the possible photocatalytic mechanisms were suggested. Furthermore, AFB₁ and degraded products (1-7) were evaluated against

SUBMITTER: Wang YD 

PROVIDER: S-EPMC8654243 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature