Double-μPeriscope, a tool for multilayer optical recordings, optogenetic stimulations or both.

ABSTRACT: Intelligent behavior and cognitive functions in mammals depend on cortical microcircuits made up of a variety of excitatory and inhibitory cells that form a forest-like complex across six layers. Mechanistic understanding of cortical microcircuits requires both manipulation and monitoring of multiple layers and interactions between them. However, existing techniques are limited as to simultaneous monitoring and stimulation at different depths without damaging a large volume of cortical tissue. Here, we present a relatively simple and versatile method for delivering light to any two cortical layers simultaneously. The method uses a tiny optical probe consisting of two microprisms mounted on a single shaft. We demonstrate the versatility of the probe in three sets of experiments: first, two

SUBMITTER: Suzuki M 

PROVIDER: S-EPMC8654370 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature