Surface display of carbonic anhydrase on Escherichia coli for CO₂ capture and mineralization.

ABSTRACT: Mineralization catalyzed by carbonic anhydrase (CA) is one of the most promising technologies for capturing CO₂. In this work, Escherichia coli BL21(DE3) was used as the host, and the N-terminus of ice nucleation protein (INPN) was used as the carrier protein. Different fusion patterns and vectors were used to construct CA surface display systems for α-carbonic anhydrase (HPCA) from Helicobacter pylori 26695 and α-carbonic anhydrase (SazCA) from Sulfurihydrogenibium azorense. The surface display system in which HPCA was fused with INPN via a flexible linker and intermediate repeat sequences showed higher whole-cell enzyme activity, while the enzyme activity of the SazCA expression system was significantly higher than that of the HPCA expression system

SUBMITTER: Zhu Y 

PROVIDER: S-EPMC8654698 | biostudies-literature | 2022 Mar

REPOSITORIES: biostudies-literature