Actinomycetes of secondary metabolite producers from mangrove sediments, Central Java, Indonesia.

ABSTRACT:

Background and aim

Actinomycetes are a group of Gram-positive bacteria with a fungus-like morphology. Their natural habitat encompasses terrestrial and water areas, including mangrove ecosystems. This study aimed to assess the PKS and NRPS genes as the producers of secondary metabolites and to determine the target bacterial species using molecular DNA tests.

Materials and methods

In this study, we isolated bacteria from sediment samples from mangrove forests located on Karimunjawa Islands and in Semarang city, purified bacteria, screened for antibacterial activity, extracted bacterial DNA, amplified the NRPS gene, detected and amplified the PKS-I and PKS-II genes, amplified and sequenced the 16S rRNA, processed molecular data, and simulated

SUBMITTER: Setyati WA 

PROVIDER: S-EPMC8654771 | biostudies-literature | 2021 Oct

REPOSITORIES: biostudies-literature