Depletion of METTL3 alters cellular and extracellular levels of miRNAs containing m⁶A consensus sequences.

ABSTRACT: Extracellular vesicles (EVs) are capable of transferring cargo from donor to recipient cells, but precisely how cargo content is regulated for export is mostly unknown. For miRNA cargo, we previously showed that when compared to isogenic colorectal cancer (CRC) cells expressing wild-type KRAS, a distinct subset of miRNAs are differentially enriched in EVs from KRAS mutant active CRC cells, with miR-100 being one of the most enriched. The mechanisms that could explain how miR-100 and other miRNAs are differentially exported into EVs have not been fully elucidated. Here, we tested the effect of N⁶-methyladenosine (m⁶A) modification on miRNA export into EVs by depletion of METTL3 and ALKBH5, a writer and eraser of m⁶A modification, respectively.

SUBMITTER: Abner JJ 

PROVIDER: S-EPMC8654799 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature