Design of a methotrexate-controlled chemical dimerization system and its use in bio-electronic devices.

ABSTRACT: Natural evolution produced polypeptides that selectively recognize chemical entities and their polymers, ranging from ions to proteins and nucleic acids. Such selective interactions serve as entry points to biological signaling and metabolic pathways. The ability to engineer artificial versions of such entry points is a key goal of synthetic biology, bioengineering and bioelectronics. We set out to map the optimal strategy for developing artificial small molecule:protein complexes that function as chemically induced dimerization (CID) systems. Using several starting points, we evolved CID systems controlled by a therapeutic drug methotrexate. Biophysical and structural analysis of methotrexate-controlled CID system reveals the critical role played by drug-induced conformational change in l

SUBMITTER: Guo Z 

PROVIDER: S-EPMC8654847 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature