Modulation of TCR signalling components occurs prior to positive selection and lineage commitment in iNKT cells.

ABSTRACT: iNKT cells play a critical role in controlling the strength and character of adaptive and innate immune responses. Their unique functional characteristics are induced by a transcriptional program initiated by positive selection mediated by CD1d expressed by CD4⁺CD8⁺ (double positive, DP) thymocytes. Here, using a novel Vα14 TCR transgenic strain bearing greatly expanded numbers of CD24^hiCD44^loNKT cells, we examined transcriptional events in four immature thymic iNKT cell subsets. A transcriptional regulatory network approach identified transcriptional changes in proximal components of the TCR signalling cascade in DP NKT cells. Subsequently, positive and negative selection, and lineage commitment, occurred at the transition from DP NKT to CD4 NKT

SUBMITTER: Dinh XT 

PROVIDER: S-EPMC8655039 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature