Construction of transgenic detection system of Brassica napus L. based on single nucleotide polymorphism chip.

ABSTRACT: Brassica napus L. is a vital oil crop in China. As auxiliary tools for rapeseed breeding, transgenic technologies play a considerable role in heterosis, variety improvement, and pest resistance. Research on transgenic detection technologies is of great significance for the introduction, supervision, and development of transgenic rapeseed in China. However, the transgenic detection methods currently in use are complex and time-consuming, with low output. A single nucleotide polymorphism (SNP) chip can effectively overcome such limitations. In the present study, we collected 40 transgenic elements and designed 291 probes. The probe sequences were submitted to Illumina Company, and the Infinium chip technology was used to prepare SNP chips. In the present Brassica napus transgen

SUBMITTER: Zhou E 

PROVIDER: S-EPMC8655060 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature