Robust, fiducial-free drift correction for super-resolution imaging.
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ABSTRACT:
SUBMITTER: Wester MJ
PROVIDER: S-EPMC8655078 | biostudies-literature | 2021 Dec
REPOSITORIES: biostudies-literature
Publications
Robust, fiducial-free drift correction for super-resolution imaging.
Wester Michael J MJ Schodt David J DJ Mazloom-Farsibaf Hanieh H Fazel Mohamadreza M Pallikkuth Sandeep S Lidke Keith A KA
Scientific reports 20211208 1
We describe a robust, fiducial-free method of drift correction for use in single molecule localization-based super-resolution methods. The method combines periodic 3D registration of the sample using brightfield images with a fast post-processing algorithm that corrects residual registration errors and drift between registration events. The method is robust to low numbers of collected localizations, requires no specialized hardware, and provides stability and drift correction for an indefinite t ...[more]
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