Establishment of a Reproducible Ischemic Stroke Model in Nestin-GFP Mice with High Survival Rates.

ABSTRACT: An accumulation of evidence shows that endogenous neural stem/progenitor cells (NSPCs) are activated following brain injury such as that suffered during ischemic stroke. To understand the expression patterns of these cells, researchers have developed mice that express an NSPC marker, Nestin, which is detectable by specific reporters such as green fluorescent protein (GFP), i.e., Nestin-GFP mice. However, the genetic background of most transgenic mice, including Nestin-GFP mice, comes from the C57BL/6 strain. Because mice from this background strain have many cerebral arterial branches and collateral vessels, they are accompanied by several major problems including variable ischemic areas and high mortality when subjected to ischemic stroke by occluding the middle cerebral artery (MCA). In

SUBMITTER: Nishie H 

PROVIDER: S-EPMC8657611 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature