Identification of Phytaspase Interactors via the Proximity-Dependent Biotin-Based Identification Approach.

ABSTRACT: Proteolytic enzymes are instrumental in various aspects of plant development, including senescence. This may be due not only to their digestive activity, which enables protein utilization, but also to fulfilling regulatory functions. Indeed, for the largest family of plant serine proteases, subtilisin-like proteases (subtilases), several members of which have been implicated in leaf and plant senescence, both non-specific proteolysis and regulatory protein processing have been documented. Here, we strived to identify the protein partners of phytaspase, a plant subtilase involved in stress-induced programmed cell death that possesses a characteristic aspartate-specific hydrolytic activity and unusual localization dynamics. A proximity-dependent biotin identification approach in Nicotiana be

SUBMITTER: Teplova AD 

PROVIDER: S-EPMC8658550 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature