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Expanding the Reactive Sulfur Metabolome: Intracellular and Efflux Measurements of Small Oxoacids of Sulfur (SOS) and H₂S in Human Primary Vascular Cell Culture.

ABSTRACT: Hydrogen sulfide (H₂S) is an endogenous signaling molecule which is important for cardiovascular health, but its mechanism of action remains poorly understood. Here, we report measurements of H₂S as well as its oxidized metabolites, termed small oxoacids of sulfur (SOS = HSOH and HOSOH), in four human primary vascular cell lines: smooth muscle and endothelial cells derived from both human arterial and coronary tissues. We use a methodology that targets small molecular weight sulfur species; mass spectrometric analysis allows for species quantification to report cellular concentrations based on an H₂S calibration curve. The production of H₂S and SOS is orders of magnitude higher in smooth muscle (nanomolar) as compared to endothelial cell lines (p

SUBMITTER: Scrivner O

PROVIDER: S-EPMC8659008 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature

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Dataset Information

Expanding the Reactive Sulfur Metabolome: Intracellular and Efflux Measurements of Small Oxoacids of Sulfur (SOS) and H₂S in Human Primary Vascular Cell Culture.

OmicsDI is part of the ELIXIR infrastructure

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Dataset Information

Expanding the Reactive Sulfur Metabolome: Intracellular and Efflux Measurements of Small Oxoacids of Sulfur (SOS) and H2S in Human Primary Vascular Cell Culture.

OmicsDI is part of the ELIXIR infrastructure

Tweets

Expanding the Reactive Sulfur Metabolome: Intracellular and Efflux Measurements of Small Oxoacids of Sulfur (SOS) and H₂S in Human Primary Vascular Cell Culture.