Ontology highlight
ABSTRACT:
SUBMITTER: Shabalina AV
PROVIDER: S-EPMC8659852 | biostudies-literature | 2021 Nov
REPOSITORIES: biostudies-literature
Shabalina Anastasiia V AV Sharko Darya O DO Glazyrin Yury E YE Bolshevich Elena A EA Dubinina Oksana V OV Kim Anastasiia M AM Veprintsev Dmitry V DV Lapin Ivan N IN Zamay Galina S GS Krat Alexey V AV Zamay Sergey S SS Svetlichnyi Valery A VA Kichkailo Anna S AS Berezovski Maxim V MV
Sensors (Basel, Switzerland) 20211125 23
We describe the preparation and characterization of an aptamer-based electrochemical sensor to lung cancer tumor markers in human blood. The highly reproducible aptamer sensing layer with a high density (up to 70% coverage) on the gold electrode was made. Electrochemical methods and confocal laser scanning microscopy were used to study the stability of the aptamer layer structure and binding ability. A new blocking agent, a thiolated oligonucleotide with an unrelated sequence, was applied to fil ...[more]