Dataset Information

Fluidics system for resolving concentration-dependent effects of dissolved gases on tissue metabolism.

ABSTRACT: Oxygen (O₂) and other dissolved gases such as the gasotransmitters H₂S, CO, and NO affect cell metabolism and function. To evaluate effects of dissolved gases on processes in tissue, we developed a fluidics system that controls dissolved gases while simultaneously measuring parameters of electron transport, metabolism, and secretory function. We use pancreatic islets, retina, and liver from rodents to highlight its ability to assess effects of O₂ and H₂S. Protocols aimed at emulating hypoxia-reperfusion conditions resolved a previously unrecognized transient spike in O₂ consumption rate (OCR) following replenishment of O₂, and tissue-specific recovery of OCR following hypoxia. The system revealed both inhibitory and stimulatory effects of H₂S on insulin secretion rate from isolated islets. The unique ability of this new system to quantify metabolic state and cell function in response to precise changes in dissolved gases provides a powerful platform for cell physiologists to study a wide range of disease states.

SUBMITTER: Kamat V

PROVIDER: S-EPMC8660022 | biostudies-literature | 2021 Nov

REPOSITORIES: biostudies-literature

ACCESS DATA

Publications

Fluidics system for resolving concentration-dependent effects of dissolved gases on tissue metabolism.

Kamat Varun V Robbings Brian M BM Jung Seung-Ryoung SR Kelly John J Hurley James B JB Bube Kenneth P KP Sweet Ian R IR

eLife 20211104

Oxygen (O2) and other dissolved gases such as the gasotransmitters H2S, CO, and NO affect cell metabolism and function. To evaluate effects of dissolved gases on processes in tissue, we developed a fluidics system that controls dissolved gases while simultaneously measuring parameters of electron transport, metabolism, and secretory function. We use pancreatic islets, retina, and liver from rodents to highlight its ability to assess effects of O2 and H2</sub ...[more]

PMID: 34734803

Similar Datasets

Project description:The sonochemical generation of hydrogen (H2) was investigated using various water/alcohol solutions under argon (Ar) 100 % in a 300 kHz sonoreactor. Five types of alcohols-methanol, ethanol, isopropanol, n-propanol, and n-butanol-were used at various concentrations (0 - 100 % v/v). The H2 generation rate in water was 0.31 μmol/min in the absence of alcohols. The H2 generation rate increased, peaked, and then decreased as the alcohol concentration increased. The concentrations used for the peak H2 generation were 5 %, 1 %, 0.5 %, 0.5 %, and 0.1 % for methanol, ethanol, isopropanol, n-propanol, and n-butanol, respectively. The highest generation rate (5.46 μmol/min) was obtained for methanol 5 % among all conditions in this study, and no H2 was detected for 100 % alcohol concentrations. The reason for the enhancement of the sonochemical H2 generation by the addition of alcohols might be due to strong scavenging effect of alcohols for sonochemically generated oxidizing radicals and vigorous reactions of alcohol molecules and their derivatives with H radicals. No significant correlations were found between the H2 generation rates and physicochemical properties of the alcohols in any of the data in this study. As alcohol concentration increased, the calorimetric power decreased. This indicates that the calorimetric power does not represent the degree of sonochemical reactions in the water/alcohol mixtures. The effect of oxygen (O2) content in the dissolved gases on the generation of H2O2 (representing sonochemical oxidation activity) and H2 (representing sonochemical reduction activity) was investigated using Ar/O2 mixtures for water, methanol 5 % and n-propanol 0.5 %. In water, the highest H2O2 generation was obtained for Ar/O2 (50:50), which is similar to previous research results. However, the H2O2 generation increased as the O2 content increased. In addition, H2 generation decreased as the O2 content increased under all liquid conditions (water, methanol, and n-propanol).

Project description:BackgroundTo figure out the efficacy, effects, safety and patient's subjective perceptions of phacoemulsification with the active-fluidics system (AFS).Patients and methodsThis was a prospective, randomized, double-masked, controlled clinical study. Age-related cataract patients were recruited and randomly assigned to the AFS group and gravity-fluidics system (GFS) group in a ratio of 1:1 to have phacoemulsification. Participants were followed up at one day, one week, one month and three months postoperatively (Chinese Clinical Trial Registry, ChiCTR2100044409).ResultsThe overall included participants were 107 finally. The total aspiration time of the AFS group was significantly less than that of the GFS group (p = .020), while no significant difference existed in cumulative dissipated energy and estimated fluid usage between the two groups. The best corrected visual acuity was significantly better in the AFS group at one day and one week postoperatively (p = .002, p = .038 respectively). The recovery of central corneal thickening and macular superficial vasculature increase was earlier in the AFS group. The central retinal thickness was significantly higher in the GFS group at one month and three months postoperatively (p = .029, p = .016 respectively). The incidence of corneal adverse events was higher in GFS group (p = .035). No serious adverse events occurred in either group. Pain scores and the scores of Cat-PROM5 questionnaire of the AFS group were significantly lower than that of the GFS group (p = .011, p = .002 respectively).ConclusionAFS improves the efficiency, effects, safety and patients' subjective perceptions of phacoemulsification compared with GFS. It is worthwhile to promote its application in cataract surgery.KEY MESSAGESThe active-fluidics system automatically detects and maintains stable intraocular pressure at the set value.The active-fluidics system improves the efficiency, effects, safety and patients' subjective perceptions in phacoemulsification.

Dataset Information

Fluidics system for resolving concentration-dependent effects of dissolved gases on tissue metabolism.

Publications

Fluidics system for resolving concentration-dependent effects of dissolved gases on tissue metabolism.

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets