Comprehensive Analysis of N⁶-Methyladenosine (m⁶A) Methylation in Neuromyelitis Optica Spectrum Disorders.

ABSTRACT: Background: N⁶-Methyladenosine (m⁶A) methylation is the most prevalent internal posttranscriptional modification on mammalian mRNA. But its role in neuromyelitis optica spectrum disorders (NMOSD) is not known. Aims: To explore the mechanism of m⁶A in NMOSD patients. Methods: This study assessed the m⁶A methylation levels in blood from two groups: NMOSD patients and healthy controls. Methylated RNA immunoprecipitation Sequencing (MeRIP-seq) and RNA-seq were performed to assess differences in m⁶A methylation between NMOSD patients and healthy controls. Ultra-high performance liquid chromatography coupled with triple quadruple mass spectrometry (UPLC-QQQ-MS) method was performed to check m⁶A level. Differen

SUBMITTER: Yang H 

PROVIDER: S-EPMC8660110 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature