Integrated stress response control of granulosa cell translation and proliferation during normal ovarian follicle development.

ABSTRACT: Mechanisms that directly control mammalian ovarian primordial follicle (PF) growth activation and the selection of individual follicles for survival are largely unknown. Follicle cells produce factors that can act as potent inducers of cellular stress during normal function. Consistent with this, we show here that normal, untreated ovarian cells, including pre-granulosa cells of dormant PFs, express phenotype and protein markers of the activated integrated stress response (ISR), including stress-specific protein translation (phospho-Serine 51 eukaryotic initiation factor 2α; P-EIF2α), active DNA damage checkpoints, and cell-cycle arrest. We further demonstrate that mRNAs upregulated in primary (growing) follicles versus arrested PFs mostly include stress-responsive upstream open reading fr

SUBMITTER: Llerena Cari E 

PROVIDER: S-EPMC8660582 | biostudies-literature | 2021 Aug

REPOSITORIES: biostudies-literature