IAPP-induced beta cell stress recapitulates the islet transcriptome in type 2 diabetes.

ABSTRACT:

Aims/hypothesis

Type 2 diabetes is characterised by islet amyloid and toxic oligomers of islet amyloid polypeptide (IAPP). We posed the questions, (1) does IAPP toxicity induce an islet response comparable to that in humans with type 2 diabetes, and if so, (2) what are the key transcriptional drivers of this response?

Methods

The islet transcriptome was evaluated in five groups of mice: beta cell specific transgenic for (1) human IAPP, (2) rodent IAPP, (3) human calpastatin, (4) human calpastatin and human IAPP, and (5) wild-type mice. RNA sequencing data was analysed by differential expression analysis and gene co-expression network analysis to establish the islet response to adaptation to an increased beta cell workload of soluble rodent IAPP, the islet response to increas

SUBMITTER: Blencowe M 

PROVIDER: S-EPMC8660728 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature