Halftone spatial frequency domain imaging enables kilohertz high-speed label-free non-contact quantitative mapping of optical properties for strongly turbid media.

ABSTRACT: The ability to quantify optical properties (i.e., absorption and scattering) of strongly turbid media has major implications on the characterization of biological tissues, fluid fields, and many others. However, there are few methods that can provide wide-field quantification of optical properties, and none is able to perform quantitative optical property imaging with high-speed (e.g., kilohertz) capabilities. Here we develop a new imaging modality termed halftone spatial frequency domain imaging (halftone-SFDI), which is approximately two orders of magnitude faster than the state-of-the-art, and provides kilohertz high-speed, label-free, non-contact, wide-field quantification for the optical properties of strongly turbid media. This method utilizes halftone binary patterned illumination t

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC8660769 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature