Changing prostaglandin E2 (PGE₂) signaling during lesional progression and exacerbation of endometriosis by inhibition of PGE₂ receptor EP2 and EP4.

ABSTRACT:

Purpose

We investigated the change, if any, in prostaglandin E2 (PGE₂) signaling in endometriotic lesions of different developmental stages in mouse. In addition, we evaluated the effect of treatment of mice with induced deep endometriosis (DE) with inhibitors of PGE₂ receptor subtypes EP2 and EP4 and metformin.

Methods

Three mouse experimentations were conducted. In Experiment 1, female Balb/C mice were induced with endometriosis or DE and were serially sacrificed after induction. Experiments 2 and 3 evaluated the effect of treatment with EP2 and EP4 inhibitors and metformin, respectively, in mice with induced DE. Immunohistochemistry analysis of COX-2, EP2, and EP4, along with the extent of lesional fibrosis, was evaluated.

Results

The immunost

SUBMITTER: Huang Q 

PROVIDER: S-EPMC8660993 | biostudies-literature | 2022 Jan

REPOSITORIES: biostudies-literature