Termination of DNA replication at Tus-ter barriers results in under-replication of template DNA.

ABSTRACT: The complete and accurate duplication of genomic information is vital to maintain genome stability in all domains of life. In Escherichia coli, replication termination, the final stage of the duplication process, is confined to the "replication fork trap" region by multiple unidirectional fork barriers formed by the binding of Tus protein to genomic ter sites. Termination typically occurs away from Tus-ter complexes, but they become part of the fork fusion process when a delay to one replisome allows the second replisome to travel more than halfway around the chromosome. In this instance, replisome progression is blocked at the nonpermissive interface of the Tus-ter complex, termination then occurs when a converging replisome meets the permissive interface. To investigate the consequences

SUBMITTER: Jameson KH 

PROVIDER: S-EPMC8661018 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature