Identification of pathways and genes associated with meniscus degeneration using bioinformatics analyses.
Ontology highlight
ABSTRACT: Objective
To explore the molecular mechanisms underlying meniscus degeneration.Methods
We performed anterior cruciate ligament resection in the Hainan Wuzhishan pig to establish a meniscus degeneration model. We applied gene chip technology to detect differentially expressed genes (DEG) in the degenerative meniscus tissues. We applied Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, core gene network, and relevant MicroRNA analyses to identify regulatory networks relevant to meniscus degeneration. We detected 893 differentially expressed genes, mainly involved in hormone production, apoptosis, and inflammation.Results
We found that MUC13, inflammatory mediator regulation of TRP channels, MDFI, and miR-335-5p may play a key role in the degenerative meniscus tissue.Conclusion
We found that meniscus degeneration involves several molecular mechanisms and provide molecular targets for future research into the disease.
SUBMITTER: Huang H
PROVIDER: S-EPMC8661235 | biostudies-literature | 2021
REPOSITORIES: biostudies-literature
Publications
Identification of pathways and genes associated with meniscus degeneration using bioinformatics analyses.
Huang Hui H Zheng Jiaxuan J Deng Ming M Fang Yehan Y Zhan Daolu D Wang Guangji G
American journal of translational research 20211115 11
<h4>Objective</h4>To explore the molecular mechanisms underlying meniscus degeneration.<h4>Methods</h4>We performed anterior cruciate ligament resection in the Hainan Wuzhishan pig to establish a meniscus degeneration model. We applied gene chip technology to detect differentially expressed genes (DEG) in the degenerative meniscus tissues. We applied Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway, core gene network, and relevant MicroRNA analyses to identify regulator ...[more]
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