RAD-R scripts: R pipeline for RAD-seq from FASTQ files to linkage maps construction and run R/QTL, operating only at copying and pasting scripts into R console.

ABSTRACT: Coupled with the reduction in sequencing costs, the number of RAD-seq analysis have been surging, generating vast genetic knowledge in relation with many crops. Specialized platforms might be intimidating to non-expert users and difficult to implement on each computer despite the growing interest in the usage of the dataset obtained by high-throughput sequencing. Therefore, RAD-R scripts were developed on Windows10 for RAD-seq analysis, allowing users who are not familiar with bioinformatics to easily analyze big sequence data. These RAD-R scripts that run a flow from raw sequence reads of F2 population for the self-fertilization plants to the linkage map construction as well as the QTL analysis can be also useful to many users with limited experience due to the simplicity of copying Excel

SUBMITTER: Seki K 

PROVIDER: S-EPMC8661492 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature