Aging Affects K_V7 Channels and Perivascular Adipose Tissue-Mediated Vascular Tone.

ABSTRACT: Aging is an independent risk factor for hypertension, cardiovascular morbidity, and mortality. However, detailed mechanisms linking aging to cardiovascular disease are unclear. We studied the aging effects on the role of perivascular adipose tissue and downstream vasoconstriction targets, voltage-dependent K_V7 channels, and their pharmacological modulators (flupirtine, retigabine, QO58, and QO58-lysine) in a murine model. We assessed vascular function of young and old mesenteric arteries in vitro using wire myography and membrane potential measurements with sharp electrodes. We also performed bulk RNA sequencing and quantitative reverse transcription-polymerase chain reaction tests in mesenteric arteries and perivascular adipose tissue to elucidate molecular underpinnings

SUBMITTER: Wang Y 

PROVIDER: S-EPMC8662361 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature