Functional analysis of a Phytophthora host-translocated effector using the yeast model system.

ABSTRACT:

Background

Phytophthora plant pathogens secrete effector proteins that are translocated into host plant cells during infection and collectively contribute to pathogenicity. A subset of these host-translocated effectors can be identified by the amino acid motif RXLR (arginine, any amino acid, leucine, arginine). Bioinformatics analysis has identified hundreds of putative RXLR effector genes in Phytophthora genomes, but the specific molecular function of most remains unknown.

Methods

Here we describe initial studies to investigate the use of Saccharomyces cerevisiae as a eukaryotic model to explore the function of Phytophthora RXLR effector proteins.

Results and conclusions

Expression of individual RXLR effectors in yeast inhibited growth, co

SUBMITTER: Wilson AC 

PROVIDER: S-EPMC8663620 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature