The small molecule SERCA activator CDN1163 increases energy metabolism in human skeletal muscle cells.

ABSTRACT:

Background and objective

A number of studies have highlighted muscle-specific mechanisms of thermogenesis involving futile cycling of Ca²⁺ driven by sarco (endo)plasmic reticulum Ca²⁺-ATPase (SERCA) and generating heat from ATP hydrolysis to be a promising strategy to counteract obesity and metabolic dysfunction. However, to the best of our knowledge, no experimental studies concerning the metabolic effects of pharmacologically targeting SERCA in human skeletal muscle cells have been reported. Thus, in the present study, we aimed to explore the effects of SERCA-activating compound, CDN1163, on energy metabolism in differentiated human skeletal muscle cells (myotubes).

Methods

In this study, we used primary myotube cultures derived from muscle biopsies o

SUBMITTER: Mengeste AM 

PROVIDER: S-EPMC8663964 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature