CeLINC, a fluorescence-based protein-protein interaction assay in Caenorhabditis elegans.

ABSTRACT: Interactions among proteins are fundamental for life and determining whether two particular proteins physically interact can be essential for fully understanding a protein's function. We present Caenorhabditis elegans light-induced coclustering (CeLINC), an optical binary protein-protein interaction assay to determine whether two proteins interact in vivo. Based on CRY2/CIB1 light-dependent oligomerization, CeLINC can rapidly and unambiguously identify protein-protein interactions between pairs of fluorescently tagged proteins. A fluorescently tagged bait protein is captured using a nanobody directed against the fluorescent protein (GFP or mCherry) and brought into artificial clusters within the cell. Colocalization of a fluorescently tagged prey protein in the cluster indicates a protein

SUBMITTER: Kroll JR 

PROVIDER: S-EPMC8664570 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature