Acute RyR1 Ca²⁺ leak enhances NADH-linked mitochondrial respiratory capacity.

ABSTRACT: Sustained ryanodine receptor (RyR) Ca²⁺ leak is associated with pathological conditions such as heart failure or skeletal muscle weakness. We report that a single session of sprint interval training (SIT), but not of moderate intensity continuous training (MICT), triggers RyR1 protein oxidation and nitrosylation leading to calstabin1 dissociation in healthy human muscle and in in vitro SIT models (simulated SIT or S-SIT). This is accompanied by decreased sarcoplasmic reticulum Ca²⁺ content, increased levels of mitochondrial oxidative phosphorylation proteins, supercomplex formation and enhanced NADH-linked mitochondrial respiratory capacity. Mechanistically, (S-)SIT increases mitochondrial Ca²⁺ uptake in mouse myotubes and muscle fibres, and decreases pyruv

SUBMITTER: Zanou N 

PROVIDER: S-EPMC8664928 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature