Multiplex PCRs for identification of Escherichia coli virulence genes.

ABSTRACT: PCRs were developed to detect 11 Escherichia coli virulence genes. Primers amplified the respective genes without cross-reaction with other genes. Specificity was maintained in multiplex reactions; excellent amplification of target genes was possible with a minimum of four multiplex reactions. These reactions successfully identified genes in E. coli from the feces of four dogs.

SUBMITTER: Pass MA 

PROVIDER: S-EPMC86652 | biostudies-literature | 2000 May

REPOSITORIES: biostudies-literature