Endoplasmic reticulum stress promotes local immunoglobulin E production in allergic rhinitis.

ABSTRACT:

Background

The role of endoplasmic reticulum (ER) stress in the pathogenesis of allergic rhinitis (AR) remains elusive.

Methods

Real-time polymerase chain reaction (RT-PCR), immunohistochemistry, and western blotting analyses were performed to detect the expression of ER stress and unfolded protein response markers: 78-kDa glucose-regulated protein (GRP78), C/EBP homologous protein (CHOP), activating transcription factor 6 (ATF6α), spliced X-box binding protein 1 (sXBP-1), and phosphorylated eukaryotic initiation factor 2α (p-eIF2α), in inferior turbinate tissue samples from patients with AR and non-AR controls. Nasal tissues from patients with AR were cultured ex vivo and treated with 4-phenylbutyric acid (4-PBA), an ER stress inhibitor.

Results

Compared to th

SUBMITTER: Liu JX 

PROVIDER: S-EPMC8665478 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature