Genetic Variability in Patients with HCV-Related Hepatocellular Carcinoma.

ABSTRACT:

Background

The present paper evaluates the genetic variability of HCV in patients with hepatocellular carcinoma (HCC).

Methods

Amino acid substitutions (aas) in NS3, NS5A and core regions were analyzed in 17 patients with HCC (Cases) and 13 without HCC (Controls), all naïve to DAAs. For the Cases, a sample of neoplastic liver tissue, non-neoplastic liver tissue and a serum sample were collected; for the Controls, a sample of liver tissue was collected. Sanger sequencing of three regions was performed using homemade protocols.

Results

Phylogenetic trees showed that there was no difference in the virus populations in the three compartments analyzed for the three HCV regions in patients with HCC. Low variability and no difference between the Cases and Controls were obser

SUBMITTER: Pisaturo M 

PROVIDER: S-EPMC8665876 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature