Processing binding data using an open-source workflow.

ABSTRACT: The thermal shift assay (TSA)-also known as differential scanning fluorimetry (DSF), thermofluor, and Tm shift-is one of the most popular biophysical screening techniques used in fragment-based ligand discovery (FBLD) to detect protein-ligand interactions. By comparing the thermal stability of a target protein in the presence and absence of a ligand, potential binders can be identified. The technique is easy to set up, has low protein consumption, and can be run on most real-time polymerase chain reaction (PCR) instruments. While data analysis is straightforward in principle, it becomes cumbersome and time-consuming when the screens involve multiple 96- or 384-well plates. There are several approaches that aim to streamline this process, but most involve proprietary software, programming k

SUBMITTER: Samuel ELG 

PROVIDER: S-EPMC8666039 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature