Proteomic discovery in sickle cell disease: Elevated neurogranin levels in children with sickle cell disease.

ABSTRACT:

Purpose

Sickle cell disease (SCD) is an inherited hemoglobinopathy that causes stroke and silent cerebral infarct (SCI). Our aim was to identify markers of brain injury in SCD.

Experimental design

Plasma proteomes were analyzed using a sequential separation approach of hemoglobin (Hb) and top abundant plasma protein depletion, followed by reverse phase separation of intact proteins, trypsin digestion, and tandem mass spectrometry. We compared plasma proteomes of children with SCD with and without SCI in the Silent Cerebral Infarct Multi-Center Clinical Trial (SIT Trial) to age-matched, healthy non-SCD controls.

Results

From the SCD group, 1172 proteins were identified. Twenty-five percent (289/1172) were solely in the SCI group. Twenty-five proteins with enriched expr

SUBMITTER: Lance EI 

PROVIDER: S-EPMC8666096 | biostudies-literature | 2021 Sep

REPOSITORIES: biostudies-literature