RNF20 affects porcine adipocyte differentiation via regulation of mitotic clonal expansion.

ABSTRACT:

Objectives

RNF20 is recognized as a main E3 ligase for monoubiquitination of histone H2B at lysine 120 (H2Bub). The critical role of RNF20 and H2Bub in various molecular events, such as DNA replication, RNA transcription, and DNA damage response, has been widely investigated and documented. However, its role in porcine adipogenesis remains unknown. In this study, we aimed to clarify the effect of RNF20 on porcine preadipocyte differentiation.

Materials and methods

Backfat tissues from fat-type pigs (Bama and Meishan) and lean-type pigs (Yorkshire and Landrace) were collected to detect the expression level of RNF20. Preadipocytes were isolated from Bama piglets and induced to differentiation. Small interfering RNAs were applied to deplete RNF20. Oil Red O staining, quantitati

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC8666272 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature