Runx3 regulates iron metabolism via modulation of BMP signalling.

ABSTRACT:

Objectives

Runx3, a member of the Runx family of transcription factors, has been studied as a tumour suppressor and key player of organ development. In a previous study, we reported differentiation failure and excessive angiogenesis in the liver of Runx3 knock-out (KO) mice. Here, we examined a function of the Runx3 in liver, especially in iron metabolism.

Methods

We performed histological and immunohistological analyses of the Runx3 KO mouse liver. RNA-sequencing analyses were performed on primary hepatocytes isolated from Runx3 conditional KO (cKO) mice. The effect of Runx3 knock-down (KD) was also investigated using siRNA-mediated KD in functional human hepatocytes and human hepatocellular carcinoma cells.

Result

We observed an iron-overloaded liver with decreased

SUBMITTER: Kim HY 

PROVIDER: S-EPMC8666273 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature