MNSFβ regulates placental development by conjugating IGF2BP2 to enhance trophoblast cell invasiveness.

ABSTRACT:

Objectives

Success in pregnancy in mammals predominantly depends on a well-developed placenta. The differentiation of invasive trophoblasts is a fundamental process of placentation, the abnormalities of which are tightly associated with pregnancy disorders including preeclampsia (PE). Monoclonal nonspecific suppressor factor beta (MNSFβ) is an immunosuppressive factor. Its conventional knockout in mice induced embryonic lethality, whereas the underlying mechanism of MNSFβ in regulating placentation and pregnancy maintenance remains to be elucidated.

Methods

Trophoblast-specific knockout of MNSFβ was generated using Cyp19-Cre mice. In situ hybridization (ISH), haematoxylin and eosin (HE), immunohistochemistry (IHC) and immunofluorescence (IF) were performed to examine the dis

SUBMITTER: Yang Q 

PROVIDER: S-EPMC8666274 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature