METTL1 promotes hepatocarcinogenesis via m⁷ G tRNA modification-dependent translation control.

ABSTRACT:

Background

N⁷ -methylguanosine (m⁷ G) modification is one of the most common transfer RNA (tRNA) modifications in humans. The precise function and molecular mechanism of m⁷ G tRNA modification in hepatocellular carcinoma (HCC) remain poorly understood.

Methods

The prognostic value and expression level of m⁷ G tRNA methyltransferase complex components methyltransferase-like protein-1 (METTL1) and WD repeat domain 4 (WDR4) in HCC were evaluated using clinical samples and TCGA data. The biological functions and mechanisms of m⁷ G tRNA modification in HCC progression were studied in vitro and in vivo using cell culture, xenograft model, knockin and knockout mouse models. The m⁷ G reduction and cleavage sequencing

SUBMITTER: Chen Z 

PROVIDER: S-EPMC8666584 | biostudies-literature | 2021 Dec

REPOSITORIES: biostudies-literature