Dataset Information

LncRNA FOXD3-AS1 Promotes Tumorigenesis of Glioma via Targeting miR-128-3p/SZRD1 Axis.

ABSTRACT:

Background

The aim of the current study was to investigate the roles of LncRNA FOXD3-AS1 (FOXD3-AS1) in the glioma progression, and its underlying mechanism of competing endogenous RNA (ceRNA) network of FOXD3-AS1/miR-128-3p/SZRD1.

Materials and methods

The FOXD3-AS1 expression and its prognostic relation were detected by bioinformatics tool. Next, glioma cell lines (HS683, U251, T98G, and SNB-19) were used to verify the FOXD3-AS1 expression. Furthermore, the roles of the FOXD3-AS1/miR-128-3p/SZRD1 axis on the glioma development in vitro and in vivo were examined.

Results

Bioinformatics analysis showed that FOXD3-AS1 was upregulated in the glioma and linked with poor prognosis. Consistently, FOXD3-AS1 level was overexpressed in the glioma cell lines (HS683 and U251). Subsequently, we verified that silencing of FOXD3-AS1 (si-FOXD3-AS1) restrained the cell proliferation, invasion, and tumor growth in vivo, and induced G0/G1 arrest, and promoted apoptosis. Further study also stated that FOXD3-AS1 interacted with miR-128-3p and SZRD1 was the target gene of miR-128-3p. Moreover, overexpression of miR-128-3p restrained the cell proliferation and metastasis of glioma, and reduced the SZRD1 level. Rescue assay illustrated that miR-128-3p inhibitor could reverse the suppressive impact of si-FOXD3-AS1 on the glioma progression. Similarly, SZRD1 overexpression could neutralize the influences of miR-128-3p mimic on glioma progression.

Conclusion

FOXD3-AS1 promoted the tumorigenesis of glioma, and exerted its function to modulate SZRD1 by targeting miR-128-3p.

SUBMITTER: Li Z

PROVIDER: S-EPMC8666723 | biostudies-literature | 2021

REPOSITORIES: biostudies-literature

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LncRNA <i>FOXD3-AS1</i> Promotes Tumorigenesis of Glioma via Targeting miR-128-3p/<i>SZRD1</i> Axis.

Li Zhang Z Li Ming M Xia Pengcheng P Wang Lili L Lu Zhiming Z

Cancer management and research 20211207

<h4>Background</h4>The aim of the current study was to investigate the roles of LncRNA <i>FOXD3-AS1</i> (<i>FOXD3-AS1</i>) in the glioma progression, and its underlying mechanism of competing endogenous RNA (ceRNA) network of <i>FOXD3-AS1</i>/miR-128-3p/<i>SZRD1</i>.<h4>Materials and methods</h4>The <i>FOXD3-AS1</i> expression and its prognostic relation were detected by bioinformatics tool. Next, glioma cell lines (HS683, U251, T98G, and SNB-19) were used to verify the <i>FOXD3-AS1</i> expressi ...[more]

PMID: 34916848

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